Zhou, Shengwang; He, Peng; Dhindwal, Sonali; Grum-Tokars, Valerie L.; Li, Ying; Parker, Kelly; Modica, Justin A.; Bleher, Reiner; dos Reis, Roberto; Zuchniarz, Joshua; Dravid, Vinayak P.; Voth, Gregory A.; Roux, Benoit; Mrksich, Milan published an article in 2021. The article was titled 《Synthesis, characterization, and simulation of four-armed megamolecules》, and you may find the article in Biomacromolecules.Name: Benzyl 2-bromoacetate The information in the text is summarized as follows:
This paper describes the synthesis, characterization, and modeling of a series of mols. having four protein domains attached to a central core. The mols. were assembled with the “”megamol.”” strategy, wherein enzymes react with their covalent inhibitors that are substituted on a linker. Three linkers were synthesized, where each had four oligo(ethylene glycol)-based arms terminated in a para-nitrophenyl phosphonate group that is a covalent inhibitor for cutinase. This enzyme is a serine hydrolase and reacts efficiently with the phosphonate to give a new ester linkage at the Ser-120 residue in the active site of the enzyme. Neg.-stain transmission electron microscopy (TEM) images confirmed the architecture of the four-armed megamols. These cutinase tetramers were also characterized by X-ray crystallog., which confirmed the active-site serine-phosphonate linkage by electron-d. maps. Mol. dynamics simulations of the tetracutinase megamols. using three different force field setups were performed and compared with the TEM observations. Using the Amberff99SB-disp + pH7 force field, the two-dimensional projection distances of the megamols. were found to agree with the measured dimensions from TEM. The study described here, which combines high-resolution characterization with mol. dynamics simulations, will lead to a comprehensive understanding of the mol. structures and dynamics for this new class of mols. In addition to this study using Benzyl 2-bromoacetate, there are many other studies that have used Benzyl 2-bromoacetate(cas: 5437-45-6Name: Benzyl 2-bromoacetate) was used in this study.
Benzyl 2-bromoacetate(cas: 5437-45-6) has been used in the alkylation of (-)-2,3-O-isopropylidene-D-threitol that afforded lipopeptide, 2-[(4R,5R)-5-({[(9H-fluoren-9-yl)methoxy]carbonylaminomethyl}-2,2-dimethyl-1,3-dioxolan-4-yl)methoxy]acetic acid.Name: Benzyl 2-bromoacetate
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary