The author of 《Funneling aromatic products of chemically depolymerized lignin into 2-pyrone-4-6-dicarboxylic acid with Novosphingobium aromaticivorans》 were Perez, Jose M.; Kontur, Wayne S.; Alherech, Manar; Coplien, Jason; Karlen, Steven D.; Stahl, Shannon S.; Donohue, Timothy J.; Noguera, Daniel R.. And the article was published in Green Chemistry in 2019. Electric Literature of C20H20BrP The author mentioned the following in the article:
Lignin is an aromatic heteropolymer found in plant biomass. Depolymerization of lignin, either through biol. or chem. means, invariably produces heterogenous mixtures of low mol. weight aromatic compounds Microbes that can metabolize lignin-derived aromatics have evolved pathways that funnel these heterogeneous mixtures into a few common intermediates before opening the aromatic ring. In this work, we engineered Novosphingobium aromaticivorans DSM12444, via targeted gene deletions, to use its native funneling pathways to simultaneously convert plant-derived aromatic compounds containing syringyl (S), guaiacyl (G), and p-hydroxyphenyl (H) aromatic units into 2-pyrone-4,6-dicarboxylic acid (PDC), a potential polyester precursor. In batch cultures containing defined media, the engineered strain converted several of these depolymerization products, including S-diketone and G-diketone (non-natural compounds specifically produced by chem. depolymerization), into PDC with yields ranging from 22% to 100%. In batch cultures containing a heterogeneous mixture of aromatic monomers derived from chem. depolymerization of poplar lignin, 59% of the measured aromatic compounds were converted to PDC. Overall, our results show that N. aromaticivorans has ideal characteristics for its use as a microbial platform for funneling heterogeneous mixtures of lignin depolymerization products into PDC or other commodity chems. The experimental part of the paper was very detailed, including the reaction process of Ethyltriphenylphosphonium bromide(cas: 1530-32-1Electric Literature of C20H20BrP)
Ethyltriphenylphosphonium bromide(cas: 1530-32-1) is a phase transfer catalyst, used to accelerate the cure of phenolic-based epoxy resins, certain fluoroelastomer resins and thermosetting powder coatings. CatOnium ETPB is also used as catalysts in the synthesis of certain organic compounds.Electric Literature of C20H20BrP
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary