Improvement of chemical analysis of antibiotics. XI. Simultaneous fluorodensitometric determination of polyether antibiotics was written by Asukabe, Hirohiko;Yoneyama, Hiromi;Mori, Yuji;Harada, Kenichi;Suzuki, Makoto;Oka, Hisao. And the article was included in Journal of Chromatography in 1987.Computed Properties of C18H11BrO The following contents are mentioned in the article:
A simultaneous determination of three polyether antibiotics, salinomycin, monensin and lasalocid, was established by silica gel and RP-18 high-performance TLC (HPTLC) using 18,19-dihydrosalinomycin and 18,19-dihydro-20-ketosalinomycin as internal standards Fluorescent derivatives of the polyethers were prepared by reaction of their Na salts with 1-bromoacetylpyrene and Kryptofix 222 in MeCN. A silica gel HPTLC plate with CCl4-EtOAc-MeCN (50:5:10) as a solvent system and a RP-18 HPTLC plate with EtOAc-MeCN-acetone-MeCN (15:2:1:55) gave satisfactory separations of the 5 pyrenacyl esters of salinomycin, monensin, lasalocid, 18,19-dihydrosalinomycin and 18,19-dihydro-20-ketosalinomycin. Under these conditions, the internal standard 18,19-dihydrosalinomycin was suitable for the simultaneous stimulation determination of salinomycin and monensin, and 18,19-dihydro-20-ketosalinomycin for lasalocid. A linear relationship was obtained between the fluorescence intensity and the amount of each antibiotic in the range 2-14 pg. The detection limit of the 3 antibiotics was 100 pg. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Computed Properties of C18H11BrO).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Most of the natural organobromine compounds are produced by marine organisms, and several brominated metabolites with antibacterial, antitumor, antiviral, and antifungal activity have been isolated from seaweed, sponges, corals, molluscs, and others. When the molecular ion is detected, the bromine and chlorine isotope patterns are very distinct, but caution is to be exercised for certain mixed chlorinated/brominated compounds, which can look similar to homohalogen patterns.Computed Properties of C18H11BrO
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary