Improvement on sample clean-up for high-performance liquid chromatographic-fluorimetric determination of diarrhetic shellfish toxins using 1-bromoacetylpyrene was written by Gonzalez, Jose C.;Vieytes, Mercedes R.;Vieites, Juan M.;Botana, Luis M.. And the article was included in Journal of Chromatography A in 1998.Electric Literature of C18H11BrO The following contents are mentioned in the article:
Okadaic acid (OA) and dinophysistoxin-2, two of the main diarrhetic shellfish toxins, can be determined by high-performance liquid chromatog. coupled to fluorimetry as pyrenacyl esters. Toxin fluorescent derivatives were obtained after quant. derivatization with 1-bromoacetylpyrene in acetonitrile. An efficient improvement in the silica gel clean-up procedure of the pyrenacyl derivatives is reported. The clean-up cartridge is washed with hexane-dichloromethane (1:1, volume/volume), dichloromethane-Et acetate (8:2, volume/volume), and finally the pyrenacyl esters were eluted with dichloromethane-methanol (9:1, volume/volume). The authors compare this procedure with other methods already described. Good results were obtained with mussels, scallops and clams. The clean-up procedure showed good robustness when checked against silica and solvents activity. Using samples of mussel hepatopancreas with an OA concentration ranging from 0 to 2 μg OA/g hepatopancreas, the inter-assay relative standard deviation ranged from 5.5 to 12.6. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Electric Literature of C18H11BrO).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Bromo compounds are employed in a variety of metal-catalyzed coupling reactions. They are also ideal candidates for the synthesis of Grignard reagents that have wide-applicability in organic synthesis. The reactivity of organobromine compounds resembles but is intermediate between the reactivity of organochlorine and organoiodine compounds. For many applications, organobromides represent a compromise of reactivity and cost.Electric Literature of C18H11BrO
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary