Fluorescence high-performance liquid chromatographic determination of free and conjugated bile acids in serum and bile using 1-bromoacetylpyrene as a prelabeling reagent was written by Kamada, Satoru;Maeda, Masako;Tsuji, Akio. And the article was included in Journal of Chromatography, Biomedical Applications in 1983.Application of 80480-15-5 The following contents are mentioned in the article:
Free and conjugated bile acids are extracted from serum or bile by using a Sep-Pak C18 cartridge and then fractionated on a piperidinohydroxypropyl-Sephadex LH 20 column. Free and glycine-conjugated bile acids are labeled with 1-bromoacetylpyrene in MeCN with dicyclohexyl-18-crown-6-ether as catalyst. Taurine-conjugated bile acids are hydrolyzed by cholylglycine hydrolase and then derivatized by the same reagent. Derivatized bile acids are separated stepwise on a reversed-phase column (Radial Pak A) using MeCN-MeOH-H2O (100:50:40) and (100:50:20) as mobile phases. The eluate is monitored by a fluorometer at 370 nm (excitation) and 440 nm (emission). Linearities of fluorescence intensities (peak heights) with the amounts of free and conjugated bile acids were obtained between 50 and 200 pmol for free bile acids and between 25 and 100 pmol for glycine-conjugated bile acids, resp. Recoveries from serum and bile samples are ≥90%. This method is sensitive, reliable, and useful for the simultaneous determination of free and conjugated bile acids in serum and bile. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5Application of 80480-15-5).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. A variety of minor organobromine compounds are found in nature, but none are biosynthesized or required by mammals. Organobromine compounds have fallen under increased scrutiny for their environmental impact. Bromine-containing agents predominate because not only are they more efficient than similar chlorine-containing species, but also the high atomic weight of bromine ensures that it is present in a high mass fraction within most organobromine compounds.Application of 80480-15-5
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary