Tetracationic Bis-Triarylborane 1,3-Butadiyne as a Combined Fluorimetric and Raman Probe for Simultaneous and Selective Sensing of Various DNA, RNA, and Proteins was written by Amini, Hashem;Ban, Zeljka;Ferger, Matthias;Lorenzen, Sabine;Rauch, Florian;Friedrich, Alexandra;Crnolatac, Ivo;Kendel, Adriana;Miljanic, Snezana;Piantanida, Ivo;Marder, Todd B.. And the article was included in Chemistry – A European Journal in 2020.HPLC of Formula: 100189-84-2 This article mentions the following:
A bis-triarylborane tetracation (4-Ar2B-3,5-Me2C6H2)-C C-C C-(3,5-Me2C6H2-4-BAr2 [Ar = (2,6-Me2-4-NMe3-C6H2)+] (24+)) shows distinctly different behavior in its fluorometric response than that of the authors′ recently published bis-triarylborane 5-(4-Ar2B-3,5-Me2C6H2)-2,2′-(C4H2S)2-5′-(3,5-Me2C6H2-4-BAr2) (34+). Single-crystal x-ray diffraction data on the neutral bis-triarylborane precursor 2 N confirm its rod-like dumbbell structure, which is important for DNA/RNA targeting and also for BSA protein binding. Fluorometric titrations with DNA/RNA/BSA revealed the very strong affinity of 24+ and indicated the importance of the properties of the linker connecting the two triarylboranes. Using the butadiyne rather than a bithiophene linker resulted in an opposite emission effect (quenching vs. enhancement), and 24+ bound to BSA 100 times stronger than 34+. Moreover, 24+ interacted strongly with ss-RNA, and CD results suggest ss-RNA chain-wrapping around the rod-like bis-triarylborane dumbbell structure like a thread around a spindle, a very unusual mode of binding of ss-RNA with small mols. Furthermore, 24+ yielded strong Raman/SERS signals, allowing DNA or protein detection at ∼10 nM concentrations The above observations, combined with low cytotoxicity, efficient human cell uptake and organelle-selective accumulation make such compounds intriguing novel lead structures for bio-oriented, dual fluorescence/Raman-based applications. In the experiment, the researchers used many compounds, for example, 2,5-Dibromo-1,3-dimethylbenzene (cas: 100189-84-2HPLC of Formula: 100189-84-2).
2,5-Dibromo-1,3-dimethylbenzene (cas: 100189-84-2) belongs to organobromine compounds. A variety of minor organobromine compounds are found in nature, but none are biosynthesized or required by mammals. Organobromine compounds have fallen under increased scrutiny for their environmental impact. When the molecular ion is detected, the bromine and chlorine isotope patterns are very distinct, but caution is to be exercised for certain mixed chlorinated/brominated compounds, which can look similar to homohalogen patterns.HPLC of Formula: 100189-84-2
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary