High-performance liquid chromatographic-fluorescence determination of human fecal bile acids was written by Ikawa, Shiro;Miyake, Mariko;Mura, Tetsuo;Ikeguchi, Masahide. And the article was included in Journal of Chromatography in 1987.COA of Formula: C18H11BrO The following contents are mentioned in the article:
A HPLC method with fluorescence detection was developed for the determination of human fecal bile acids, especially free fecal bile acids. Fecal bile acids were extracted by using an Amberlite XAD-2 column and then fractionated into 4 groups (free, glycine-conjugated, taurine-conjugated, and sulfated bile acids) on a piperidinohydroxypropyl-Sephadex LH 20 column. The free bile acid fraction and free bile acids obtained after enzymic hydrolysis and/or solvolysis of the 3 other fractions were derivatized with 1-bromoacetylpyrene and dicyclohexyl-18-crown-6-ether. The derivatized bile acids were separated stepwise on a Shim-pack CLC-ODS column with MeCN-MeOH-H2O (100:50:30) (A), (100:50:20) (B), and (100:50:0) (C) as mobile phases while changing automatically from A to C by using a solvent changer. Calibration curves of bile acid standards were linear in the range 20-400 pmol when monitored at 370 nm (excitation) and 440 nm (emission). Percent recoveries of bile acids from human feces samples were 80-95%. The method is applicable clin. and is sensitive, reliable, and useful for the detailed determination of human fecal bile acids, especially of free bile acids. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5COA of Formula: C18H11BrO).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Organo bromine compounds are versatile compounds and are widely used in diverse fields. Organo bromine derivatives are used in the dye sector, as an indicator in analytical chemistry (Bromothymol blue is a popular indicator). The principal reactions for organobromides include dehydrobromination, Grignard reactions, reductive coupling, and nucleophilic substitution.COA of Formula: C18H11BrO
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary