Carbon nanotube – protamine hybrid: evaluation of DNA cell penetration was written by Caoduro, Cecile;Kacem, Raoudha;Boukari, Khaoula;Picaud, Fabien;Brachais, Claire-Helene;Monchaud, David;Borg, Christophe;Boulahdour, Hatem;Gharbi, Tijani;Delage-Mourroux, Regis;Hervouet, Eric;Pudlo, Marc. And the article was included in Carbon in 2016.COA of Formula: C18H11BrO The following contents are mentioned in the article:
Carbon nanotubes (CNTs) represent a class of nanomaterials with important potential for biomedical and biotechnol. applications. CNT based vectorization is an emerging approach to the transport of nucleic acid through cell membrane but limited by detachment of DNA and degradation process. To increase DNA internalization, it was proved that cationic functionalized CNT was essential. In such a way, protamine efficiently used in several transfection processes is a cationic protein which was never associated to CNT. We propose here a novel nanovector based on single-walled carbon nanotubes (SWCNT) functionalized by protamine. Our results based on qPCR methods clearly indicate that protamine-SWCNT/DNA hybrids were much more successful than ammonium-SWCNT/DNA hybrids to cross cell membrane and penetrate into cells. We also studied the cellular internalization mechanism of our complexes and showed that endocytosis was strongly involved. Finally, we proposed mol. dynamic simulations to understand the formation of the protamine-SWCNT/DNA complex and to extract the interaction responsible for its deliverance upon cell membrane. This study involved multiple reactions and reactants, such as 2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5COA of Formula: C18H11BrO).
2-Bromo-1-(pyren-1-yl)ethanone (cas: 80480-15-5) belongs to organobromine compounds. Organo bromine compounds are versatile compounds and are widely used in diverse fields. Organo bromine derivatives are used in the dye sector, as an indicator in analytical chemistry (Bromothymol blue is a popular indicator). Bromine-containing agents predominate because not only are they more efficient than similar chlorine-containing species, but also the high atomic weight of bromine ensures that it is present in a high mass fraction within most organobromine compounds.COA of Formula: C18H11BrO
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary