Williams, Sierra J. published the artcileOrthogonal bioluminescent probes from disubstituted luciferins, COA of Formula: C10H16Br3N, the publication is Biochemistry (2021), 60(8), 563-572, database is CAplus and MEDLINE.
Bioluminescence imaging with luciferase-luciferin pairs is routinely used to monitor cellular functions. Multiple targets can be visualized in tandem using luciferases that process unique substrates, but only a handful of such orthogonal probes are known. Multiplexed studies require addnl. robust, light-emitting mols. In this work, we report new luciferins for orthogonal imaging that comprise disubstituted cores. These probes were found to be bright emitters with various engineered luciferases. The unique patterns of light output also provided insight into enzyme-substrate interactions necessary for productive emission. Screening studies identified mutant luciferases that could preferentially process the disubstituted analogs, enabling orthogonal imaging with existing bioluminescent reporters. Further mutational analyses revealed the origins of substrate selectivity. Collectively, this work provides insights into luciferase-luciferin features relevant to bioluminescence and expands the number of probes for multicomponent tracking.
Biochemistry published new progress about 111865-47-5. 111865-47-5 belongs to bromides-buliding-blocks, auxiliary class Benzenes, name is Mono(N,N,N-trimethyl-1-phenylmethanaminium) tribromide, and the molecular formula is C6H6N2O, COA of Formula: C10H16Br3N.
Referemce:
https://en.wikipedia.org/wiki/Bromide,
bromide – Wiktionary