From bacterial genomes to novel antibacterial agents: discovery, characterization, and antibacterial activity of compounds that bind to HI0065 (YjeE) from Haemophilus influenzae was written by Lerner, Claude G.;Hajduk, Philip J.;Wagner, Rolf;Wagenaar, Frank L.;Woodall, Charlotte;Gu, Yu-Gui;Searle, Xenia B.;Florjancic, Alan S.;Zhang, Tianyuan;Clark, Richard F.;Cooper, Curt S.;Mack, Jamey C.;Yu, Liping;Cai, Mengli;Betz, Steven F.;Chovan, Linda E.;McCall, J. Owen;Black-Schaefer, Candace L.;Kakavas, Stephan J.;Schurdak, Mark E.;Comess, Kenneth M.;Walter, Karl A.;Edalji, Rohinton;Dorwin, Sarah A.;Smith, Richard A.;Hebert, Eric J.;Harlan, John E.;Metzger, Randy E.;Merta, Philip J.;Baranowski, John L.;Coen, Michael L.;Thornewell, Susan J.;Shivakumar, Annapur G.;Saiki, Anne Y.;Soni, Niru;Bui, Mai;Balli, Darlene J.;Sanders, William J.;Nilius, Angela M.;Holzman, Thomas F.;Fesik, Stephen W.;Beutel, Bruce A.. And the article was included in Chemical Biology & Drug Design in 2007.COA of Formula: C9H8BrFO2 This article mentions the following:
As part of a fully integrated and comprehensive strategy to discover novel antibacterial agents, NMR- and mass spectrometry-based affinity selection screens were performed to identify compounds that bind to protein targets uniquely found in bacteria and encoded by genes essential for microbial viability. A biphenyl acid lead series emerged from an NMR-based screen with the Haemophilus influenzae protein HI0065, a member of a family of probable ATP-binding proteins found exclusively in eubacteria. The structure-activity relationships developed around the NMR-derived biphenyl acid lead were consistent with on-target antibacterial activity as the Staphylococcus aureus antibacterial activity of the series correlated extremely well with binding affinity to HI0065, while the correlation of binding affinity with B-cell cytotoxicity was relatively poor. Although further studies are needed to conclusively establish the mode of action of the biphenyl series, these compounds represent novel leads that can serve as the basis for the development of novel antibacterial agents that appear to work via an unprecedented mechanism of action. Overall, these results support the genomics-driven hypothesis that targeting bacterial essential gene products that are not present in eukaryotic cells can identify novel antibacterial agents. In the experiment, the researchers used many compounds, for example, Ethyl 4-bromo-2-fluorobenzoate (cas: 474709-71-2COA of Formula: C9H8BrFO2).
Ethyl 4-bromo-2-fluorobenzoate (cas: 474709-71-2) belongs to organobromine compounds. Bromo compounds are employed in a variety of metal-catalyzed coupling reactions. They are also ideal candidates for the synthesis of Grignard reagents that have wide-applicability in organic synthesis. alpha-Bromoesters are employed in the Reformatsky reaction for the synthesis of beta-hydroxyesters. The reactivity of organobromine compounds resembles but is intermediate between the reactivity of organochlorine and organoiodine compounds. For many applications, organobromides represent a compromise of reactivity and cost.COA of Formula: C9H8BrFO2
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary