Kinetic studies of mammalian and microbial cholesterol esterases in homogeneous aqueous solutions was written by Lynn, K. R.;Chuaqui, C. A.;Clevette-Radford, N. A.. And the article was included in Bioorganic Chemistry in 1982.Application of 35065-86-2 This article mentions the following:
The kinetics of 1 microbial and 2 mammalian (bovine and porcine pancreatic) cholesterol esterase have been examined using a variety of aryl acetates in homogeneous solution The mammalian enzymes behaved identically but differed somewhat from that of microbial origin. Electronic and hydrophobic substrate characteristics had no effect on enzyme activity. Taurocholic acid was without effect on the microbial enzyme; at low concentrations it inhibited the mammalian enzyme, but when present in millimolar amounts notable increases in rate were discerned, attributable to the detergent effect on the enzyme. In the experiment, the researchers used many compounds, for example, 3-Bromophenyl acetate (cas: 35065-86-2Application of 35065-86-2).
3-Bromophenyl acetate (cas: 35065-86-2) belongs to organobromine compounds. Many of the organo bromine compounds are relatively nonpolar. Bromine is more electronegative than carbon (2.8 vs 2.5) and hence the carbon in a carbon–bromine bond is electrophilic in nature. Bromine-containing agents predominate because not only are they more efficient than similar chlorine-containing species, but also the high atomic weight of bromine ensures that it is present in a high mass fraction within most organobromine compounds.Application of 35065-86-2
Referemce:
Bromide – Wikipedia,
bromide – Wiktionary