Let¡¯s face it, organic chemistry can seem difficult to learn. Especially from a beginner¡¯s point of view. Like 2067-33-6, Name is 5-Bromopentanoic acid. In a document, author is Zhu Yaozu, introducing its new discovery. HPLC of Formula: C5H9BrO2.
Betulinic acid inhibits glioma cell viability by down-regulation of NF-kappa B and enhancement of apoptosis
Purpose: To determine the inhibitory potential of betulinic acid on pm-survival signaling pathway in glioblastoma. Methods: Changes in viabilities of glioma cells and primary astrocytes were measured using 3-(4, 5dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Apoptotic changes were analyzed using Hoechst 33342 staining and Annexin V-FITC/PI kits. Western blotting was used for assaying the protein expressions of various pro-apoptotic and anti-apoptotic factors. Results: The proliferative potential of U87MG and A172 cells were significantly reduced on treatment with betulinic acid in a concentration- and time-dependent manner. Treatment with betulinic acid at a dose of 8.75 mu g/mL increased apoptosis in U87MG and A172 cells to 41.8 +/- 0.5 and 48.8 +/- 0.5%, respectively (p < 0.05). Betulinic acid significantly decreased intracellular levels of NF kappa B p65 and suppressed levels of survivin, XIAP and Bcl-2 in U87MG and A172 cells (p < 0.05). However, betulinic acid significantly increased the levels of Bax and activated caspase-9 and caspase-3 in U87MG and A172 cells (p < 0.05). Conclusion: Betulinic acid inhibited the proliferation of U87MG and A172 glioblastoma cells and mediated their apoptosis. There is need for in vivo studies for validation of the therapeutic potential of betulinic acid as an anti-glioblastoma drug. I hope this article can help some friends in scientific research. I am very proud of our efforts over the past few months and hope to 2067-33-6 help many people in the next few years. HPLC of Formula: C5H9BrO2.